About this person

Skills:

Molecular biology:

  • Experience on  DNA and RNA extraction from different tissues, cDNA synthesis, cloning, probe making methodologies (cRNA) as well as PCR.   
  • Experience on gene quantification using Real Time qPCR and genes distribution using In Situ Hybridisation (ISH) methodology, DAB and fluorescence Immunohistochemistry. 
  • Laser micro dissection: advanced tool to isolate and capture of specific cells for gene expression.
  • DNA analysis by micro-satellite mapping techniques.                                      
  • Measurement of  circulating hormone level using  ELISAs.

Histology: Experience on  preparation of histological samples (sampling, fixation, embedding, cryosectioning)

Cellular Biology: organ culture of fish gills, total RNA extraction from cells

Instruments: FAST- prep FP 120, BIO 101 Thermo Savant, Cryostat Leica CM 3050S Agilent 2100 Bio analyzer, InsituPro,  nanodrop 1000ND Spectrophotometer.

Selected publications:

Grassie, Cairsty; Braithwaite, Victoria Anne; Nilsson, Jonatan; Nilsen, Tom Ole; Teien, Hans-Christian; Handeland, Sigurd Olav; Stefansson, Sigurd Olav; Tronci, Valentina; Gorissen, Marnix; Flik, Gert; Ebbesson, Lars O.E. Aluminum exposure impacts brain plasticity and behavior in Atlantic salmon (Salmo salar). Journal of Experimental Biology 2013 ;Volum 216.(16) s. 3148-3155

Ebbesson, Lars O.E.; Nilsen, Tom Ole; Helvik, Jon Vidar; Tronci, Valentina; Stefansson, Sigurd Olav. Corticotropin-Releasing Factor Neurogenesis during Midlife Development in Salmon: Genetic, Environmental and Thyroid Hormone Regulation. Journal of neuroendocrinology (Print) 2011 ;Volum 23.(8) s. 733-741

Skjæraasen, Jon Egil; Nilsen, Trygve; Meager, Justin James; Herbert, Neill A.; Moberg, Olav; Tronci, Valentina; Johansen, Torild; Salvanes, Anne Gro Vea.
Hypoxic avoidance behaviour in cod (Gadus morhua L.): The effect of temperature and haemoglobin genotype. Journal of Experimental Marine Biology and Ecology 2008 ;Volum 358.(1) s. 70-77

Publications

Articles

Other publications

  • Vindas, Marco Antonio; Gorissen, Marnix; Höglund, Erik; Flik, G.; Tronci, Valentina; Damsgård, Børge; Thörnqvist, Per-Ove; Nilsen, Tom Ole; Winberg, Svante; Øverli, Øyvind; Ebbesson, Lars O.E. How do individuals cope with stress? A neuroendocrine substrate for proactive and reactive coping styles in fish. 2016.
  • Denker, Elsa; Nilsen, Tom Ole; Tronci, Valentina; Hazlerigg, David; Ebbesson, Lars O.E. Turning light into hormonal signalling: mapping the melatonin receptive cells in the brain of the juvenile Atlantic salmon (Salmo salar), and circadian and seasonal variations through smoltification. 2016.
See all publications in CRIStin

Ongoing projects

All projects
cp: 2017-10-21 04:18:03