NorZymeD - Enzyme development for Norwegian biomass

Project Department: Uni Research Environment (group: Applied Biotechnology) period: 22.11.12 - 22.11.18

Project results

Publication from Uni Research:

  • Bjerga GEK, Arsın H, Larsen Ø, Puntervoll P, Kleivdal HT. A rapid solubility-optimized screening procedure for recombinant subtilisins in E. coli. J Biotechnol [Internet]. Elsevier B.V.; 2016 Mar 20;222(February 2007):38–46. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26854945

About the project

Enzyme development for Norwegian biomass – mining Norwegian biodiversity for seizing opportunities in the bio-based economy

The basis for a national bio-economy lies in the development of a biorefining industry enabled by enzyme tools that are tailored for Norwegian biomass and business opportunities. This project focuses on developing enzymes and processes for biomasses and value chains where Norway has clear competitive advantages, namely lignocellulosics and marine co-products from fisheries and aquaculture. Starting at unique biodiversities of the Arctic, hot vents and gut microbiomes, the project will build generic competence by establishing a national “enzyme development pipeline”.

Through national complementarity and distribution of tasks, we will combine expertise on thermophilic enzymes in Bergen and cold-adapted enzymes in Tromsø (UiT), with expertise in enzyme engineering and applied enzymology in Ås (NMBU), fermentation and screening facilities at Trondheim (SINTEF), and structural characterization in Tromsø (UIT).

We will develop generic technology competence in four work packages, focusing on metagenomie sampling (WP 1) screening and characterization of candidate enzymes (WP2, WP3), enzyme engineering (WP4) and larger-scale production for industrial trials (WP2). The project workflow will follow the value creation chain all the way to industrial trials. This is underscored by the involvement of two Norwegian companies with a strong R&D focus, which has made them leaders in lignocellulosic (WPS) and marine co-product biorefining (WP6). By linking enzyme development in two different value chains (blue and green), the project will unleash considerable synergies. Ethical, legal and societal aspects (ELSA) will be pursued in a dedicated work package (WP8). Expanding the interest to explore biocatalytic opportunities will be an important task for the management group (WP7, WP9).

Secondary objectives:

  • A national pipeline for identification, optimization and production of industrially useful enzymes
  • A technology platform for stabilization and immobilization of enzymes
  • Targeted development of enzymes for lignocellulose processing
  • Targeted development of enzymes for processing of marine biomass
  • Identify en develop novel opportunities for enzymatic biomass processing
  • Dealing with ethial, societal and legal issues
  • A national arena for industrial biocatalysis and biorefining

Expected output:

  • Top national competence in fundamental enzymology and enzyme technology
  • Value-added products from marine by-products – peptones, lipids, feed, nucleotides
  • Sustainable, profitable and downscalable bioprocessing options for Norwegian biomass
  • Value-added products from lignocellulosic biomass – lignin, polymers, fish feed, chemical building blocks
  • Second generation biofuels
  • IP on enzymes and enzymatic processes
cp: 2017-12-15 16:18:04